Coding

Part:BBa_K5374011:Design

Designed by: Langning Xu   Group: iGEM24_TestDaily-I-Beijing   (2024-09-12)


CBD MMPs-EGFP.A collagen-binding domain from matrix metalloproteinases (MMPs) with strong affinity f


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 484
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 484
    Illegal NheI site found at 922
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 484
    Illegal BglII site found at 897
    Illegal BamHI site found at 288
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 484
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 484
    Illegal NgoMIV site found at 859
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1565


Design Notes

None


Source

EGFP = Enhanced green fluorescent protein which can be used as a reporter gene.

References